Blending the might of artificial intelligence with seasoned expertise, we meticulously selected fragments of potential biological significance from the human fibronectin sequence. We have identified a highly potent fragment through various in vitro assessments and mechanistic studies, and further validated the function via a series of rigorous clinical studies. We optimize the microbial strain expression to significantly improve the yield of target products. Following a meticulous four-stage purification protocol, we achieved the epitome of ingredient – Prorylia™ FN.